Package: PlateVision 0.1.0
PlateVision: Automated qPCR Analysis and Visual Quality Control
Directly pipes raw quantitative PCR (qPCR) machine outputs into downstream analyses using the comparative Ct (Delta-Delta Ct) method described by Livak and Schmittgen (2001) <doi:10.1006/meth.2001.1262>. Streamlines the workflow from 'Excel' export to publication-ready plots. Integrates unique visual quality control by reconstructing 96-well plate heatmaps, allowing users to instantly detect pipetting errors, edge effects, and outliers. Key features include automated error propagation, laboratory master mix calculations, and generation of bar charts and volcano plots.
Authors:
PlateVision_0.1.0.tar.gz
PlateVision_0.1.0.zip(r-4.7-any)PlateVision_0.1.0.zip(r-4.6-any)PlateVision_0.1.0.zip(r-4.5-any)
PlateVision_0.1.0.tgz(r-4.6-any)PlateVision_0.1.0.tgz(r-4.5-any)
PlateVision_0.1.0.tar.gz(r-4.7-any)PlateVision_0.1.0.tar.gz(r-4.6-any)
PlateVision_0.1.0.tgz(r-4.6-emscripten)
manual.pdf |manual.html✨
DESCRIPTION
card.svg |card.png
PlateVision/json (API)
| # Install 'PlateVision' in R: |
| install.packages('PlateVision', repos = c('https://probablyshubham.r-universe.dev', 'https://cloud.r-project.org')) |
This package does not link to any Github/Gitlab/R-forge repository. No issue tracker or development information is available.
Last updated from:9829849b18. Checks:9 OK. Indexed: yes.
| Target | Result | Time | Files | Syslog |
|---|---|---|---|---|
| linux-devel | OK | 125 | ||
| source / vignettes | OK | 200 | ||
| linux-release | OK | 215 | ||
| macos-release | OK | 84 | ||
| macos-oldrel | OK | 115 | ||
| windows-devel | OK | 70 | ||
| windows-release | OK | 76 | ||
| windows-oldrel | OK | 73 | ||
| wasm-release | OK | 105 |
Exports:calculate_ddctcalculate_reagentscheck_replicatesimport_plateplot_barsplot_volcanoview_plate
Dependencies:askpassbase64encbslibcachemcellrangerclicpp11crayoncrosstalkcurldata.tabledigestdplyrevaluatefarverfastmapfontawesomefsgenericsggplot2gluegtablehighrhmshtmltoolshtmlwidgetshttrisobandjquerylibjsonliteknitrlabelinglaterlazyevallifecyclemagrittrmemoisemimeopensslotelpillarpkgconfigplotlyprettyunitsprogresspromisespurrrR6rappdirsRColorBrewerRcppreadxlrematchrlangrmarkdownS7sassscalesstringistringrsystibbletidyrtidyselecttinytexutf8vctrsviridisLitewithrxfunyaml
Readme and manuals
Help Manual
| Help page | Topics |
|---|---|
| Calculate ddCt Statistics | calculate_ddct |
| Calculate Master Mix Reagents | calculate_reagents |
| Check Technical Replicates | check_replicates |
| Import and Merge PCR Data | import_plate |
| Plot Fold Change (Bar Chart) | plot_bars |
| Plot Volcano (Log2FC vs P-value) | plot_volcano |
| Visualize Plate Layout (Heatmap) | view_plate |